I just wanted to make sure that I understand how the z-projections that I’ve been using work. I think I have a handle on the basics - a ‘Maximum Projection’ image uses the brightest voxel at each xy point of the stack, ‘Average Intensity’ uses an average of value of all of those voxels, and ‘Sum Slice’ uses a sum of values, correct? Are the latter two calculated literally by just the arithmatic average or sum of the voxel values along a single z-axis point, or is there more to it than that?

Thanks in advance,

Peter G. Werner

Postgraduate Student, MSc, Microscopy and Imaging

NUI, Galway, 2018-2019