Zeiss phases photos edge

3D Zeiss structure fluorescence phases
By using structure fluorescence phases, I got 5 phases photos in each x-y plane.
Firstly, I need to verify the edge of my sample on each X-Y plane, but I don’t know how can I find the edge.
The difference among 5 phases photos are the grid position, the grid is the edge of my sample in fact. But there are too many other interference infos in the photos,
Which plungins can I use to solve this problem, and thank you for your help!!!

Welcome to the forum, @jing_zhang!

Sorry that this post has been sitting without reply for so long.

Maybe you can upload some example image or screenshot illustrating your issue? This would greatly improve the chance of someone here on the forum being able to help you.