Hi all, I have some zebrafish images where I have to quantify the GFP expression of heart,liver,kidney and brain altogether. I tried something like this:
a) Cropped the area of my interest (from head to trunk region).
b) subtracted background: rolling ball radius=50 pixels.
c) Min threshold value ranged from 30-100. Maximum threshold value was 255.
I am new at imageJ. Can anyone suggest me how to subtract background value and keep minimum threshold value same for all images OR am I doing it in right way?