I’m testing CellProfiler (Mac version 2.0) to analyze stacks of images taken with a confocal scope on MetaMorph, and some seemingly identical stacks give me this error message using the LoadImages module:
Error while processing LoadImages:
ZCT size vs image count mismatch (sizeZ=20, sizeZ=1, sizeT=1, total=21)
The stacks were not altered from the MetaMorph output. Changing the file name didn’t change the error. I got the error with both “stk movies” and “tif, tiff, flex movies”. I couldn’t figure out the problem after a cursory review of the code. I couldn’t attach the movies because they are greater than 10 MB. I cropped the movies in ImageJ to get them under 10 MB, but I no longer got the error message with the cropped movie. I can upload the movies somewhere else if you need to look at them.