can you please advise on working with z-stacks. I have .oib z-stack files, and I’m importing in the usual way with Metadata -> Extract from image file headers (updating metadata and table buttons), NamesAndTypes -> Images matching rules, but then I get into the troubles.
I select a rule to match the channel 0 (even though I have only one), and I click + to add the Image Is Stack Frame option, and give it a name (I’ve tried with and without the channel as I have only 0, but the same question arises). Help suggests “In the “Image set matching method” setting, select “Metadata”.”, but for a single channel z-stack you don’t get that option. I continue all the same.
In Groups I use FileLocation and it shows the 3 files I’ve dragged in, grouped into 3 as I’d expect with a count of 5 each (the number of z in each stack), so all looks good.
When I go to perform a MakeProjection and run through the Test Mode it appears to make a projection for all of the individual 5 z’s of each of the 3 files… Is there a way to get it to process the Analysis modules once for each file? Right now I don’t know if it’s working or if it’s just the way it works through.