I want to segment yeast cells in bright field images. I attached the pipeline I am using at the moment. It works with binary images and the results are good for easy images where the cells are isolated. Unfortunately the results get worse when the images get more complicated for example when there are lots of cells in one image or when the cell shape starts to change in budding processes.
Do you have any idea how to improve the segmentation in these images? Maybe working with grey scale images? Or would edge detection make any difference?
Brightfield_analysis0910.cp (8.89 KB)