In a previous post I brought up an x-y alignment issue. Mark added the issue to the tracker: github.com/CellProfiler/CellProfiler/issues/758
I was wondering if I can somehow bypass the issue in the meantime, but increasing the size of the nucleus after primary segmentation.
At the moment I identify the secondary object with Distance - N. Can I somehow make it identify the secondary object by starting X pixel outside the primary object, thus enlarging the nuclear mask? Or just make the secondary object start farther from the border of the nucleus? (same effect)
scanr_v1_noAlign.cp (13.7 KB)