I have used the volume fraction plugin of BoneJ to estimate the volume fraction in the stack of the micro-CT image of an open-cell foam. In the stack, there are 100 slices, and the pixel size is 2.25597 um. When I run the volume fraction plugin in BoneJ, although the value of volume ratio seems reasonable, the value of the total volume is WAY lower than what I expect. The dimension of the images is 1800x1200 pixels, and I have 100 slices in the stack, so I expect the total volume to be 1800x1200x100x2.25597x2.25597x2.25597 um3 = 2 480 011 595,39 um3. However, the boneJ result for the total volume is 97638.38 um3. Do you think I am missing something or the boneJ output is wrong ?