I have question about inensities measurement. I am measuring Alexa intensity in cell mask from GFP channel. The images are 12-bit TIF saved as 16-bit.
So I am doing rescaling E (min/max) But Ijust found that the inensities measurements do not correspond to the image intensities.
In Alexa image I have some saturated pixels (rescaled intensity 1), the pixels are within object mask, but in the output table my max intensity
is 0.6. Could you help me?
I also found that there is something strange in rescale intensity module help for method E - settings 0, 0.0625, 0, 0, 1, 1. For me it did not work, I had to
se 0, 0.0625, 0, 1, 0, 1. Is it correct?
Thanks a lot.
I am using Windows XP, Cell Profile version 1.0.6031.
P.S. I could attached only pipeline and two .zip compressed pictures, so GFP picture is missing. I can send it separately.
DAPI - n000000.tif.zip (1.38 MB)
Alexa 647 - n000000.tif.zip (1.85 MB)
tubulin3.pipe.mat (1.45 KB)