For a long time, I’m struggling in figuring out, how to use CP for images where there is no nuclear dye used.
It has only one channel: GFP (green).
The images are basically from a time-lapse live cell imaging. However, I have saved each frame as a single tif file to be used in CP.
I have attached two of the 6 images here (jpg format).
So, is it possible to study these images?
If so, what pipeline we can use?
Below is the illumination correction pipeline that I developed.
SmoothOrEnhance (smoothing method: Enhance BrightRoundSpeckles (Tophat Filter))
RescaleIntensity (Rescaling method. (S) Stretch the image (0 to 1))
After this, when I run the actual pipeline for object identification and measurements, it couldn’t identify the primary objects, hence no result in the end.
PLz, help me in this issue.
Thanks for yr constant support.