On a Windows 8.1 box I downloaded the latest CellProfiler and the “Human Cells” example pipeline.
Everything seems to be going OK until I press “Analyze Images”. In a few seconds I see “Pipeline error”. Here’s what was copied to the clipboard:
Traceback (most recent call last):
File “analysis_worker.py”, line 367, in do_job
File “cellprofiler\measurements.pyc”, line 1767, in load_measurements_from_buffer
File “tempfile.pyc”, line 293, in mkstemp
File “tempfile.pyc”, line 228, in _mkstemp_inner
OSError: [Errno 2] No such file or directory
Is this some sort of permission problem in setting up a temporary file?
Thanks for any clue about how to troubleshoot this.