Question.tif (3.3 MB)
I am new to this forum and I am hoping someone can help me understand this image.
It should be (nearly) all black, as it is my control (cells without any fluorescent treatment) + the illumination time was extremely short.
However, if I open it with Fiji/ImageJ, it is displayed as grey. In other programs (for example with the windows photo viewer it looks completely black). Why is that?
Also, the histogram looks as if it was messed with, but I didn’t change any settings.
Could someone please help me understand this problem?
If I open images taken with the exact same settings, but a longer illumination time, I don’t encounter these problems.
I want to measure the (raw) integrated density, but I am worried that the results will be wrong if the histogram is not displayed correctly.