We are trying to separate myocyte nuclei from fibroblasts using a stain for alpha-actin. Using a Lower Quartile Intensity measurement for the alpha-actin channel we achieve some selectivity for myocyte nuclei as shown in the attached image:
We would like to use a texture measurement as an additional way to select for myocytes by picking out the banded z-line structure. However, the Gabor measure does not seem to be correlated with z-line structure as indicated in the following image.
I used a scale of 3 pixels and a Gabor threshold = mean + std = 3.05
What options and values should we try in order to use the texture module to pick out the z-line structure?
Thank you for your assistance
Gabor-test.cppipe (29.1 KB)
(For some reason I was unable to upload original image. It may be too large to post.)