This is a very unusual thing that I have observed.
We are using DAPI as nuclear staining and two other proteins with different dyes.
Upon running CP, when I looked at total integrated Intensity for blue (DAPI) in nucleus & propcytoplasm, i found that this intensity is not only present in propcytoplasm but also, its much higher than the nucleus.
Its a serious concern for the lab. What could be the reason?
Plz help us and let me know for any information required !!