I have the following two images:
What I want to do is to detect the redness area and quantify it.
For that I use the following step:
run("Duplicate...", " "); run("HSB Stack"); setThreshold(131, 255); run("Analyze Particles...", "display exclude include summarize stack");
The detected redness area looks like this:
Detected area image: patient-01-005-minus4wks
Detected area image: patient-01-005-Day0
And the stats looks like this:
As you can see in the stats the total area of Hue and Saturation in minus4wks data is much larger than in Day0 patient. Hue, 12 times larger and Saturation 4.6 times larger.
But when we look at the detected area by eye, clearly Day0 area is at least almost the same if not covered more area than minus4wks.
What is the cause of the discrepancy and how can I resolve that?