I am using Confocal Laser Scanning Microscopy, ( Leica TCS SP8) to do 3D imaging of surfactant distributions on nonwoven polymer fiber. I treat my samples in a way that makes the surfactants fluoresce. It is this fluorescence, (associated with presence of surfactants) which I would like to quantify. My aim is to visualise the surfactants in 3D and also to quantify the amount of surfactants present in my samples for aging studies purposes. Please can any one recommend what is the best image analysis software for me to use? All the image analysis software tools freely available such as IFiji, Icy, CellProfiler, QuPath… have been developed for the analysis of biological samples. My samples are polymeric nonwoven fibers modified with surfactants. I have no programming knowledge and I am not sure how to justify using any of these softwares…Anyone knows of alternative image analysis softwares better suited for me please?