I’ve come across a weird result and it took me some time to figure out how to supress it.
- Take any image
- Apply correctilluminationcalculate, use background, rescale illumination function to YES.
- Either Correctilluminationapply or Imagemath with divide operation.
- Apply a mask that is bigger than 50% of the total area of image.
- The masked output is a lot brighter than the image after illumination correction. You can see on the same opened window that the masked image is a lot brighter, but when you hover over the same regions, the intensity value at the bottom of the window say the same number!!!
If you use rescale illumination fucntion to NO, and correctilluminationapply or imagemath with SUBTRACT, this does not happen.
Why? Am i doing something weird?