I am new in CellProfiler and I am trying to obtain informations about the blood vessels in immunofluorescent tissue sections. I would like to know the % of area covered (per field), the number of vessels (per field), the diameter of the vessels (on this parameter I have concern mainly for the vessel with a “sling” shape as I don’t know which parameters can gave me the right result between Min Feret Diameter and Minor Axis Length), I would also try to count how many vessels have an elongated shape and how many have a “sling” shape or a hole in the middle. I am sending 2 images as example.
I wrote a pipeline but I also have issues with the pipeline as it doesn’t save the images (even if it says it does).
ValeriaVessels.cpproj (431.8 KB)