Hello everyone,

I’m currently trying to measure 3D heart volumes on light sheet microscopy stack. However, I’m encountering some problems, and I need some help please.

Here is the story : when I acquire the images, I have something like that :

First step, to calculate the volume of the heart “muscles”, I threshold the stack to obtain something like that :

And thanks to an advice of @ThomasBoudier that I have found on an other site, I am able to calculate the volume of the thresholded area using the histogram and calibration (as I know my voxel size). So, this is great.

Now, here come the real problem. I want to measure the volume of the ventricule cavities (left one, right one, and both of them at the same time). Though I have tried many techniques, I am not able to find a solution to isolate on the stack the cavities of interest and caculate their volumes. Do you have any solution, please?

Thank you in advance for your help, and have a nice day,

Nabil Nicolas.