I am struggling to identify an endothelial marker (Claudin-5) in a mouse brain section of a stroke. There is a degree of background specifically in the stroke area when stained with Cl-5 using DAB. I am able to analyse the other areas of the brain easily enough, but I am finding difficulty with the area below as there is also staining of haemosiderin (yellow circular objects in the below image).
I have looked at a similar post relating to pig brain and hematoxylin, but the solution to that doesn’t quite work for us (Help in identifying vessels).
Would anybody be able to suggest an appropriate pipeline that would help identify the vascular stain only?
Thanks in advance