I´m trying to identify stained nuclei (HTX) and also are of positively stained CD68 in our tissue samples. Though it seems that the images varies due to different background staining and therefore are hard to quantify. I have tried to change the intensity and diameter, though the problem is that when the settings fits one Picture another Picture is detected with a lot of background. Is there any possibility of detecting all of the Pictures in spite of different backgrounds?
I have attached some example Pictures and also the pipline i have used.
CD68.cpproj (100 KB)