Dear ImageJ forum,
My team and I would like to measure vacuole count and/or their diameters across different time frames. The vacuoles in the image above are the black round circles inside a cell (e.g. the ~5-6 vacuoles that form a “ghost face” in the top left section of the image. I am relatively new to Fiji, and I tried using the Trackmate plugin, but it couldn’t detect the vacuoles, I tried using the manual tracking but it is too slow and tedious. We also tried counting them manually but this also could become a large source of error from user to user. Is there something else that you could recommend us to use to make these measurements? Any help is highly appreciated! Thank you !