I’ll preface my post by saying I am a FIJI novice–which is why I’m reaching out. I’m interested in tracking cells for a few .avi files I have. However, I have been unable to make the trackmate plugin properly identify my cells. I’ve tried LoG/DoG and many threshold settings, but trackmate consistently misidentifies cells. I’m starting to believe its an importation/file type error or perhaps its something simple that I’m overlooking. If anyone has experience tracking colored cells I’d really appreciate your advice. I realize its likely I haven’t provided enough information in my post to diagnose a problem, so please feel free to ask me for more information as well. Thanks!