Is there a way by which the single feature classifier can be used multiple times to classify objects in an image?
Can the above be done through a script?
I am trying to calculate H-score for cells which I would like to detect using the “cell + membrane detection” method. However, there is no way to calculate the H-score in that method.
Additionally, “cell + membrane” detection segments regions that do not have a nucleus (correspondingly there is no nucleus hematoxylin or DAB measurement for these objects).
Therefore, to restrict the analysis to objects that only have a nucleus, I would first like to labels objects with a certain minimum nuclear hematoxylin value as “valid cells” and then would like to threshold the valid cells as high, medium or low based on their cell DAB mean value.
Using the single feature classifier, I am able to classify the valid cells. However, when I try to further classify valid cells I am not able to reliably run the method more than twice. More specifically. the method resets the objects to valid cells.
Is there a better way to do this? Any suggestions are greatly appreciated!