Using PCA to perform a 3D reconstruction

Hello everyone, I have data of several 3d vertebra structures, and what I have done so far is:

  • calculate parameters as: Thickness, Connectivity and Space distance using Bonej on several ROI.

  • Calculate PCA model of those parameters and ROI

  • Choose one ROI and convert it to DRR

  • Compare the similarity between this DRR and an X-ray Radio

what I want now if the difference is too big, is that I have to make some changes on the 3D structure and convert it again to DRR and compare it with the x-ray Radio, but i don’t know how to use my PCA model for this propose and how to change my 3D structure.

Any advise please? Many Thanks