Due to my studies of Medicine, I am recently working on a science Project, which requires evaluating the effect of a certain substance on the synaptogenesis of hippocampal rat cells.
I have collected a huge amount of data, more specific photos of my neurons which now need to be evaluated - their synapses need to be counted.
I wanted to use the NeuronJ Plugin for Fiji but there have been two major problems to it, which is why I need your help!
After installing the Plugin NeuronJ and colouring my dendrits the manuel wants me to open a Plugin called “SynapCountJ”, but I cannot find a working download link (or even the named Plugin under “Plugins” in Fiji.
In order to compare the different number of synapses, I somehow need to measure the same length of every dendrite - I have no idea how to do so as there is no way of marking a certain area (of the dendrite) and measure at the same time.
I´d be thrilled about some help!