I have a IF pictures of cells stained for my protein of interest which are also stained with DAPI for nuclei. I was wondering if how to calculate the amount of my protein of interest that is found in the nucleus or cytoplasm. I guess I would need to determine the pixel density/intensity found 1) within the segmented nuclei or 2) outside of the segmented nuclei region but not in the background either (i.e. the cytoplasm).
I saw in another post @VolkerH commented:
Does anyone know how this can be done? My experience with these types of image analyses is almost 0.
Also, I don’t necessarily need the information on an individual cell basis, just more so for any given picture (that i have 50-100 cells) the percentage of protein X that is nuclear and the percentage that is cytoplasmic.