I have some images from a FISH experiment in which cells contain 2 different types of signals (for my locus of interest and a reference) in different colours/channels. I’ve attached a couple of images as examples.
I’m scoring this manually, which is relatively straightforward. I think the point tool in FIJI (2.0.0. on Mac) could be useful to quickly count and save a copy of the analysis. However, as far as I’ve seen I can only create a series of points. Does anyone have a workaround or suggestion to be able to create 2 point groups (with different point types and counters) on the same image? I can obviously do it in separate copies of the image but it’d be useful to have both overlayed.
I don’t know if this something the multi-point option could be used for - I haven’t been able to make much sense of it and the tool seems to have changed compared to what’s described in the user manual I’ve found online (from FIJI 1.46.).
Any tips would be much appreciated.