Using cytoplasm staining to segment cells


I have a mixture of mono- and binucleated cells in population.
Thus, I need to introduce cytoplasmic membrane staining to distinguish between 2N and 4N cells. Somehow I can’t build a pipeline to segment cells nicely. I am struggling with it for few days by now.

It would be nice if you could give me some tips.

I tried to analyze only by nuclear staining but this segmentation also was not super reliable…
I would be really thankful for any tips.


Sorry we missed this query. Did you find a solution?