Using Color Deconvolution


I have trichrome sections stained and was wondering if my steps are correct.

  • Run Masson’s Trichrome color deconvolution plug-in
  • Set measurements to area, area fraction, limit to threshold
  • Threshold red filtered image for % of muscle using an algorithm OR by manual thresholding

Thank you.

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There is a trichrome vector, but you should determine your own vector to make sure that it matches your images.

If i determine my own vector, do i use roi?

That is clearly explained here:

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