Sample image and/or macro code
I’m trying to use CellProfiler Analyst to count the number of cells in a series of images. I have 2 channels for each image (DAPI and Alexa). My cells tend to be found in clusters and I’m trying to use DAPI as a seed to separate the cells labeled with Alexa.
So far, I’ve had pretty good success using just a CellProfiler pipeline, but I think CPA could improve my accuracy. I want to be able to process my images in CellProfiler and then use CPA to further refine identification and count the total number of Alexa and DAPI positive cells.
I’ve tried to be thorough in CellProfiler, but I can’t CPA to load anything other than blank image segments (no cells on them). I’d appreciate any help - thanks in advance!