HI, First, thanks for this terrific tool!!!
I have been working with CP3 for some time now and I find it very useful and powerful for our needs. However, I have been struggling with some issues…
For example, I would like to use CP to analyse FRET time lapse recordings (2 channels for FRET (donor & acceptor) per confocal image, every given interval --> a huge tiff file).
So, in order to get offline analysis of FRET dynamics, I would like CP to measure fluorescence of objects for the entire file, take care of the background, etc. That I can do.
But then, I would like CP to take a subset of the images within the file (I can specify which images i.e. t = 1 to 5) identified as baseline and isolate values for these images and then do some calculation (I can take care of the calculation part too).
So, to make a big story short… Can CP use a subset of images (or frames) within a file, identify them and use the data later in a pipeline? And if so, how?
I tried in the “NamesAndTypes” module (using criteria like t < 5 to differently name images) or by grouping to identify let say images from time 1 to 5, but it doesn’t work. I have no experience in coding or python whatsoever…
I would greatly appreciate if someone could help me!
And actually, the ability to use a subset of images within a file would be very helpful to several other applications I have…
Thanks a lot!!