Use cells from one image to analyze another image

Hi have two files that show intestine stained with two different stains. What I would like to be able to do is to detect the blue stained regions and then look at the same spot on the red stained file and determine the mean or median intensity of those objects. I have uploaded the pipeline that I am currently working with as well as the sample images. I am able to split an image into the different color channels and get intensity values for one image but am not sure how to use the coordinates for one image to generate the intensity values for the other image. Any help would be greatly appreciated. Thank you!




pipeline.cppipe (16.2 KB)

You can do this in the MeasureObjectIntensity module. There, you can add multiple images to measure the object intensity from. So if you add the red channel, then the module will make intensity measurements of the nuclei from both channels.
-Mark

Thank you Mark!