I have tried a few times to limit the upper bound of thresholding when identifying primary objects in tissue slices. This is convenient to eliminate artefacts, as they tend to be saturated. Somehow,this does not seem to change anything to the segmentation. I have tried lower and upper bounds of 0.19 and 0.191 and can segment all the objects up to 1. The lower bound definitely works the upper one does not really do anything. Did I misunderstand something?