Unwanted merged foci

Hi,
I am a first time user of cell profiler. I changed the speckle sample a little bit and seems working OK. However, it merges the spots and recognizes a foci which I don’t think is a foci (please see the left green one in image2; image2 is zoom-in of image1). Could you help me to figure out the problem?

Thanks,
Rong




Hi Rong,

You may want to try changing the lower limit of the threshold in IdentifyPrimaryObjects to a value that excludes these dim false positives. Alternately, you can increase the threshold correction factor to achieve a similar result.

Regards,
-Mark