I downloaded the demonstration file for UntangleWorms. While the test data (2 images) work perfectly, the trained model does not give me the results I want. Therefore, I tried training for my own dataset. The diameter of the objects I am interested in are larger than the predefined sizes in the demonstration. I changed them to min = 40 and max = 100.
The output shows the worm nicely identified…
drive.google.com/folderview?id= … sp=sharing
Traceback (most recent call last):
File “cellprofiler\pipeline.pyc”, line 2307, in post_group
File “cellprofiler\modules\untangleworms.pyc”, line 727, in post_group
File “numpy\linalg\linalg.pyc”, line 445, in inv
File “numpy\linalg\linalg.pyc”, line 328, in solve
LinAlgError: Singular matrix
What am I doing wrong?