I am using CellProfiler to analyze pictures of C elegans acquired on a high-content imager. My pipeline looks good until it reaches UntangleWorms. I thought maybe my training set was bad so I switched to train mode and still the module will not work.
Basically, when it gets to UntangleWorms, the time to completion begins to increase as does memory usage. If I do not stop it, the program will run out of memory and crash. When I do stop it, I have to restart the program to clear the memory, otherwise it will continue to increase even if the pipeline is not running.
I had a similar problem before with ‘infinite’ worms at the edges of the images, but I have altered the masking to eliminate that problem.