It’s working now. I made sure that the CorrectIlluminationCacluate module took input directly from the Legacy input modules. But I have another problem. Why do I have a blank image after correcting for illumination? Before I used the correct illumination module, I used to have objects identified by the identify objects module but the numbers where so low in some and very high in others because of intensity differences. Please what and where have I gone wrong?
Kindly find attached my pipeline, screenshot, the raw images, the raw images in gray scale and the identified blobs. The images are consecutive images (25 and 26) and I expect them both to have roughly the same amount of blobs/crystals.
Also, which will be better to use as input for the CorrectIlluminationCalculate module? The raw images or the gray scale images?
Thank you as I look forward to hearing from you.
5_3.cppipe (17.9 KB)