Hello! I am new to Fiji, and I am following a protocol where I am supposed to adjust the threshold of my TIF images to binarise them. However, every time I input my lower/upper threshold and press “set”, the threshold levels jump to a random value (usually 255), and no matter how much I try to change the threshold from the sliding bar or type it in, it does not work.
I am not familiar with coding my own macros or doing anything remotely related to coding, so I am unable to understand any of the solutions given by others to similar this problem people have written about on this forum. However, I did try to update my Fiji software to see if it was a bug, and I have also tried to downgrade to all the older available versions, but to no avail. Could anyone please help me out? I really need this resolved urgently. Thank you.