I’ve been using QuPath to analyze my DAB staining for some time now and have always had great success with my positive cell detection. I am now trying to analyze fluorescent images but it wouldn’t pick up on any cells. When I select an annotation to quantify, it begins the process, then quickly finishes without ever having identified a single cell even though I selected the correct image type, “fluorescent”.
The following post helped me to identify an issue with my pixel sizes: Trouble with Positive Cell Detection in Tiff files - #6 by Research_Associate. As you can see, the scale bar in that image is in mm rather than um, and it turns out this was the case in my images as well. However, what I can’t figure out is how to correct this issue. In my case, when I click the image tab, it says my metadata is not changed.
Can anyone please offer some insight into how to correct the pixel size of my images?
Thank you in advance!!