Thank you so much for your reply and advise Ellen.
I’ve gotten a few ultrasound images like the one uploaded to compare the vascular density on each tumour, in order to do it I want to measure both blobs: the red and the blue, since each of them are vessels.
I have tried using Threshold in greys-scale (or 8 bite) but either the red or the blue they get grey and not white, which is not working for me because I need them white so I can measure them, since they are what I need to mesure.
Before, for measuring the vascular density in other images (like immunofluorescence tiff images) what I’ve been doing is to use threshold and then deciding and specific area to measure in different places, to later compare them with the samples I’ve gotten of each tumour.
But with ultrasound images I honestly got no experience and I’ve read it is possible to measure with imageJ, but did not get how, so I was wondering whether here I could find someone with experience with it who could explain me.
Pd: I’ve tried my best being specific, sorry if I didn’t get to succeed, I’m new at this but I really try to learn about it.
Thank you once again.