I am using Cellprofiler 2.4.0 for the quantification of adipocytes and crown-like-structures in fat tissue. For the quantification of the crown-like-structures I wanted to include the module IdentifyObjectsManually as the structures are hard to distinguish from normal adipocytes. Though, I get an error when it reaches this module in the pipeline. The following error is displayed:
When selecting continue processing I get the following window:
What can I do to fix this? It even has this error when I analyze the image in a seperate pipeline including only this module.
Thanks in advance!