My name is Fernanda and I am a research engineer at UCL. I’ve been trying to use Cell profiler to analyse my trypan blue exclusion pictures for three types of cells:
- Viable round cells
- Non-viable/dead (stained blue) cells
- Viable elongated cells
I am new to Cell profiler and by playing around with different pipelines I have managed to identify cells but I can seem to be able to differentiate from one type to another. Basically, I do not know how to tell Cell profile to pick those coloured cells as non-viable/dead. I haven’t got round to elongated yet. Any help I would appreciate. Attached a sample of one of my pictures.