I’m trying to design a pipeline in which I can:
Identify the center of a nucleus
Superimpose a circle with a fixed radius (say, 50 microns) on the image, using the center of the nucleus as the center of the circle
I am hoping to do this in order to trace neurites out a given distance from the center of the cell.
Does anyone know if this is possible in CellProfiler, or is it better to employ ImageJ for steps like this?