Hello, I am trying to use CellProfiler to count cells, and also count fluorescent particles inside the cells. And then finally to categorize the cells in to 6 categories: # cells in image, how many cells did not consume particles, how many cells consumed 1, 2-5, 6-10, and >10.
I have gotten as far as making the fluorescent particles a primary object, cells a secondary. Although I do not know how to find the pixel size of my cells. I also think ‘MeasureObjectNeighbors’ could have something to do with it but I am unsure.
I usually have to spend many hours going through the images myself, and then somebody told me about this program. Any help would be appreciated. I have included an image as an example.