Good day image experts,
I am having trouble setting the threshold on an image to be used to count particles. The particles in the image are protein particles that were stained with a fluorescent dye.
I was successful at thresholding the image and count the particles ONCE, but when I tried to duplicate the steps I get a blank image and only one or two particles that are being counted.
original image no processing:
with insert original.tif (7.9 MB)
image with threshold:
with insert original with filter.tif (3.9 MB)
image after analyse particles:
blank analyze particles.tif (3.9 MB)
These are the steps I took and it worked one time, but I haven’t being able to duplicate the results.
- Gaussian blur to remove noise from image
- LUT invert
- Analyze particles.
Any guidance will be appreciated as well as explanation as to why it might have worked once and not continued to work.