Trouble Segmenting Stem Cells

CellProfiler Team:

So I am able to segment and analyze the nucleus of these cells using a nucleus stain, but I am having a lot of trouble trying to segment the cells. Any ideas? Do you think imaging at a higher magnification would help to elucidate the cell boundaries? Does anyone have any good staining suggestions for stem cells?

Thanks!

Sincerely,

Chris
MIT Class of 2013



Hi Chris,

It may not be that satisfying, but see #3 in the FAQ:

http://forum.image.sc/t/cellprofiler-2-0-faq/12699/1

Best,
David

Word of advice: use fluorescent images…