Trouble identifying primary objects - high cell density


I have some trouble identifying my primary objects; they are very dense and are difficult to separate based on intensity, or shape - could any of you point me towards the best thresholding setting within the Identify Primary Objects module?
The life database is here:

Thanks a million :slight_smile:

Hi there,

Itโ€™s indeed a challenging case. You can try do the following:

Here is the example pipeline: lif.cpproj (643.1 KB)

Good luck.

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Hi Minh,

Itโ€™s working a treat, thank you!

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