I am very new to using CellProfiler and have just started playing around with it, but have not found a way to have the program properly identify the muscle cells shown from the image.
The closest I’ve come is producing cell-looking shapes as secondary objects based on propagation, with the primary objects as the nuclei (peripherally located at cell borders).
However, the objects identified do not seem to correspond to actual cell shapes at all, and I have not been able to improve on automatic threshold settings.
I’ve attached a screenshot of the images produced from object identification.
I’m continuing to try to educate myself from the tutorials, etc, but would very much appreciate any advice.