I’m wondering if this is possible in imagej? I have two stacks of images: a stack with images of a big organism and a stack with images of a small organism. I’ve already marked cells of interest throughout the stack as multipoint ROIs, different in each slice, in both organisms and now I want to use an affine transform to map the smaller organism onto the bigger organism.
Using BigWarp and the question here I can take one slice from each stack and map, via an affine transform, the smaller organism onto the bigger organism. (I’m having trouble exporting the image though).
I’m not really interested in the image of the organism itself though, only the ROIs.
So I have two questions:
- Is there a way of just loading a stack of ROIs without the original image?
- Is there a way of transforming one image stack (ideally this would be a stack of just ROIs) onto another image stack?
Thank you for any help, it’s really appreciated!